u937 cell line  (Highveld Biological Pty)

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: PCA,principal component analysis of differentially expressed genes in PRF-activated U937 and THP-1 macrophages. The plot depicts the sample distribution in a two-dimensional space defined by the first and second principal components of the covariance matrix. Gene expression values were normalized and expressed as logCPM, log counts per million.

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Gene Expression

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: Volcano plot analysis of differentially expressed genes in U937 and THP-1 macrophages treated with PRF. Volcano plots illustrate upregulated (red) and downregulated (blue) genes in U937 and THP-1 cells following PRF exposure. The annotated data points represent the 50 genes with the greatest Euclidean (Manhattan) distance from the origin that exceed the defined significance thresholds (dashed lines). Thresholds were set at –log 10 (p) ≥ 2.0 and |log 2 fold change| ≥ 3.0.

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: Heatmap of differentially expressed genes in U937 and THP-1 macrophages exposed to PRF lysates. The heatmap visualizes the 395 upregulated and 372 downregulated genes in U937 cells, and the 116 upregulated and 149 downregulated genes in THP-1 cells, following exposure to PRF lysates. Differentially expressed genes were defined by an adjusted p-value < 0.05. The dataset integrates transcriptional data from three independent PRF preparations. Color gradients indicate relative expression levels, with red representing upregulation and blue representing downregulation; color intensity reflects the magnitude of the expression change.

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: Venn analysis of differentially expressed genes in U937 and THP-1 macrophages treated with PRF lysates. Venn diagrams show the overlap of upregulated and downregulated genes between U937 and THP-1 cells after exposure to PRF lysates (adjusted p < 0.05). A total of 28 genes were commonly upregulated, and 20 genes were commonly downregulated in both cell lines.

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: Functional enrichment analysis of upregulated genes in U937 macrophages stimulated with PRF lysates. Functional enrichment analysis, also known as over-representation analysis (ORA), was performed using the g:Profiler web-based platform. The plot depicts the top significantly enriched pathways derived from Gene Ontology and other integrated databases. Pathways are ranked and labeled numerically according to significance. P-values were adjusted for multiple testing using the Benjamini–Hochberg correction method (Padj).

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Functional Assay, Derivative Assay, Labeling

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: Functional enrichment analysis of downregulated genes in U937 macrophages stimulated with PRF lysates. Functional enrichment analysis (over-representation analysis, ORA) was performed using the g:Profiler web-based platform. The figure displays the top significantly enriched pathways identified from Gene Ontology and other integrated databases, ranked and labeled numerically by significance. p-values were adjusted for multiple testing using the Benjamini–Hochberg correction method (Padj).

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Functional Assay, Labeling

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: RT–qPCR validation of selected genes in U937 macrophages stimulated with PRF lysates. U937 macrophages were exposed to PRF lysates for 48 hours before quantitative RT–PCR analysis. Data represent the median of four independent experiments and two gene panels (A, B) .

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Biomarker Discovery

Journal: Frontiers in Immunology

Article Title: Transcriptomic characterization of platelet-rich fibrin-induced macrophage responses identifies U937 cells as a sensitive bioassay

doi: 10.3389/fimmu.2026.1722342

Figure Lengend Snippet: RT–qPCR and ELISA analysis of inflammatory gene expression in U937 and THP-1 macrophages stimulated with PRF lysates. U937 (A, B) and THP-1 (C, D) macrophages were exposed to PRF lysates for 48 hours before quantitative RT–PCR analysis. ELISA quantified CXCL8 protein levels in the corresponding cell culture supernatants. Data represent the median of independent experiments.

Article Snippet: The human monocytic cell lines U937 (CRL-1593.2) and THP-1 (TIB-202) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Gene Expression, Cell Culture